Biofilm formation by estuarine microorganisms and their bioactivitiesWEBINAR
by
BIT Mesra
About Speaker:
Dr Joydeep Mukherjee
Prof. Dr. Joydeep Mukherjee obtained his bachelors degree in Pharmaceutical Sciences from Birla Institute of Technology (Mesra, India) in 1990. He also possesses a masters degree in Biotechnology in 1992 and Ph.D. in the same area from Jadavpur University (Kolkata, India) in 1997. During his doctoral work Prof. Mukherjee was a German Academic Exchange Fellow in the University of Hannover, Germany and later a post doctoral fellow from 1998 to 2000 in the same university having fellowships of the German Research Foundation and the Daimler and Benz Foundation. Later he completed another post doctoral research stay at the Australian Institute of Marine Science, Townsville in 2007. Prof. Mukherjee joined Jadavpur University in 2000 and presently he holds a Professor position and is also the Director of the School of Environmental Studies of Jadavpur University. He held a special associate professorship position of the University Grants Commission tenable in the School of Environmental Studies of Jadavpur University from 2013 till 2020. Prof. Mukherjee is currently teaching Biochemical Engineering, Bioremediation and Environmental Health and Toxicology to M. Tech. (Environmental Biotechnology) students. His research interests include microbial diversity of the Sundarbans (the world’s largest tidal mangrove forest), biofilm formation, bioplastic production, marine sponge pathogenesis and recycling of rural slaughterhouse wastes. Prof. Mukherjee has research collaborations with Germany, Australia and Canada. He has published 90 international research papers and obtained two patents. Fifteen students have obtained their doctoral degrees under his supervision and placed in academic and scientific positions in India and abroad.
Abstract of Talk:
Biofilm Formation by Estuarine Microorganisms and Their Bioactivities
In the fluctuating intertidal environment, biofilms provide protection and may structure a range of bioprocesses. Against this background, we set out to find novel methods of cultivation of estuarine microorganisms. Our patented device (the conico-cylindrical flask, USP 8,945,917 B2) was used for the cultivation of estuarine microorganisms from different geographical regions of the world, each producing a bioactive compound (pigment, enzyme, vitamin or antimicrobial). The vessel facilitates easy removal of slides which can subsequently be observed through confocal laser scanning microscopy. The conico-cylindrical flask further allows identification of the crucial parameters (vessel surface hydrophobicity/ hydrophilicity, growth, biofilm formation and aeration) influencing productivity of estuarine biofilm-forming microbes, knowledge of which in the initial stages of process development can assist informed decisions at later stages of bioprocess development. The rotating disc bioreactor with discs rotating at 2 revolutions per day mimicked the niche-intertidal environment and supported growth and biofilm formation by estuarine microorganisms. This design has been noted as the first model reactor system for in vitro process simulation of the intertidal/ estuarine environment (Zhang Y, Arends JBA, Van de Wiele T, Boon N: Bioreactor technology in marine microbiology: From design to future application: Biotechnology Advances 29, 312–321, 2011). Recently, biofilm-enhancing cultivation approach was applied for assessing the potential of multispecies intertidal biofilms for the removal of polycyclic aromatic hydrocarbons from estuarine water of the Sundarbans (the world’s largest tidal mangrove forest). Our publications and patents in this area of research are:
- Enhanced production of antimicrobial compounds by three salt-tolerant actinobacterial strains isolated from the Sundarbans in a niche-mimic bioreactor: Sarkar et al: Marine Biotechnology, 10, 518-526, 200
- Antibiotic production by a marine isolate (MS310) in an ultra low speed rotating disk bioreactor: Sarkar et al: Biotechnology and Bioprocess Engineering, 14 (6) 775-780, 2009
- Production of a potentially novel antimicrobial compound by a biofilm-forming marine Streptomyces sp. in a niche-mimic rotating disk bioreactor: Sarkar et al: Bioprocess and Biosystems Engineering, 33 (2), 207-217, 2010
- Enhanced protease production in a polymethylmethacrylate conico-cylindrical flask by two biofilm-forming bacteria: Sarkar et al: Bioresource Technology, 102, 1849-1855, 2011
- A novel conico-cylindrical flask aids easy identification of critical process parameters for cultivation of marine bacteria: Mitra et al: Applied Microbiology and Biotechnology, 90, 321–330, 2011
- Cellulase and xylanase activity in relation to biofilm formation by two intertidal filamentous fungi in a novel polymethylmethacrylate conico-cylindrical flask: Mitra et al: Bioprocess and Biosystems Engineering 34, 1087-1101, 2011
- Induced biofilm cultivation enhances riboflavin production by an intertidally derived Candida famata: Mitra et al: Applied Biochemistry and Biotechnology, 166, 1991-2006, 2012
- Enhanced biotransformation of fluoranthene by intertidally-derived Cunnighamella elegans through biofilm-based and niche-mimic cultivations: Mitra et al: Applied and Environmental Microbiology, 79, 7922-7930, 2013
- Enhanced biofilm formation and melanin synthesis by the oyster settlement-promoting Shewanella colwelliana is related to hydrophobic surface and simulated intertidal environment: Mitra et al: The Journal of Bioadhesion and Biofilm Research 31 (3), 283-296, 2015
- Assessment of polycyclic aromatic hydrocarbon contamination in the Sundarbans, the world’s largest tidal mangrove forest and indigenous microbial mixed biofilm-based removal of the contaminants: Balu et al. Environmental Pollution, 266, 115270, 2020
- Enhanced surface area conico-cylindrical flask (ES-CCF) for biofilm cultivation: US Patent No.: US 8,945,917 B2, Date of patent: February 3rd, 2015. Inventors: Sarkar et al.
- Device and method of providing enhanced surface area for growth and attachment of biofilms/ biological cells in a modified cylindrical flask: Datta et al. Indian patent application no. 202031044832, First examination report responded, 2022
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An incredible Journey of 50 years of the Department
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16th -18th December 2022
Department of Pharmaceutical Science & Technology
Birla Institute of Technology, Mesra, Ranchi 835215 (JH), India