Speaker
Description
Verbascoside is a phenylethanoid glycoside that shows several biological activities including anti-diabetic and anti-inflammatory activity. The drug increases keratinocyte migration due to which it can inhibit inflammation, hence it can be considered as a potential candidate for wound healing when applied topically. But the biggest challenge with verbascoside is its low skin permeability which can be vanquished by delivering it through biomaterials. This study was aimed to assess the wound healing potential of green synthesized silver nanoparticle (AgNP). The AgNP was fabricated and optimized using Verbascoside using QbD approach and characterized using various analytical techniques like UV, FTIR, DLS, XRD, and FESEM. Further, AgNPs were coated with coating agents like β-cyclodextrin (β-CD), sodium dodecyl sulfate (SDS), and polyethylene glycol (PEG) 6000. The coated nanoparticles were analytically assessed to see the efficiency of coating. The coated and uncoated AgNPs were loaded into hydrogel as it improves tissue localization, minimizes burst release of nanoparticles, and controls the sequential drug release all while preserving the structural integrity of the nanoparticle. Hydrogel was prepared from dispersions of the different AgNPs with the help of gelling agents like Chitosan, Sodium carboxymethyl cellulose, Sodium alginate, Hydroxypropylmethyl cellulose, and Pluronic F-127. These formulations were later evaluated to understand their spreadability, viscosity, and in vitro drug release. A comparative study of the coated, uncoated nanoparticles, and the loaded formulations were conducted with respect to their antimicrobial activity.