Speaker
Description
Abstract:
Aim: A simple, sensitive, rapid, reliable, and cost-effective HPTLC method for simultaneous estimation of "Amlodipine" and "Moxonidine" in bulk and pharmaceutical dosage form was developed and validated. Since combined drug formulations are so important in medical science these days, it is critical to standardize the product using modern analytical techniques.
Methodology: This study provides an opportunity for standardisation of combined marketed formulations. Methanolic solutions of "Amlodipine" and "Moxonidine" were chromatographed on 20cm x 10cm (layer thickness 250µm, aluminum-backed silica gel 60 F254 plate, E. Merck, Darmstadt, Germany) with solvent system Tri-ethylamine: Methanol (9:1.5). Calibration plots were established showing the dependence of response on the amount chromatographed.
Result: The linear regression analysis data for the calibration plots revealed a good linear relationship with R² = 0.999 for Amlodipine peak area between concentration ranges 2-10µg/ml and R² = 0.998 for Moxonidine peak area between concentration ranges 1-5µg/ml. CAMAG TLC scanner, densitometer scanning at λ= 237nm for Amlodipine and λ= 251nm for Moxonidine by using deuterium lamp, and WINCAT software were used for detection and quantification. Amlodipine's standard Rf value of 0.57 and Moxonidine's standard Rf value of 0.85 were matched with the sample.
Conclusion: The method was validated in accordance with the ICH guidelines and can be used to validate and standardise other related pharmaceutical products.
Keywords: HPTLC, Amlodipine, Moxonidine, Simultaneous estimation