Speaker
Description
Rasmita Jena1, 2, Durga Madhab Kar2
1 School of Pharmacy and Life Sciences, Centurion University of Technology and Management, Bhubaneswar, Odisha, 752050
2 Department of Pharmacology, School of Pharmaceutical Sciences
Siksha ‘O’ Anusandhan Deemed to be University, Kalinga Nagar, Bhubaneswar, Odisha, 751003
Background: Aporosa octandra (Euphorbiaceae) is an ethnomedicinal evergreen shrub locally known as Massania. Tribal people are mostly using different plant parts for several aliments like jaundice, stomach ulcer, skin infection, colic fever, rheumatism, fracture, cuts, wounds and itches.
Aim: This study was carried out to evaluate the antimicrobial activity along with the phytochemical screening of various leaf extracts of A. octandra.
Methods: The dried leaves powder was defatted by using petroleum ether and extracted by cold maceration using methanol and distilled water as solvent. The crude methanol and aqueous extract were subjected to qualitative phytochemical screening as per the standard protocol to determine the presence of secondary metabolites. Further, the antimicrobial activity of bioactive extracts of different concentrations (200µg/ml and 100µg/ml) was evaluated against different multi-drug resistance bacteria by the agar well diffusion method.
Results: The phytochemical analysis of potent extract reveals that methanol extract of A. octandra showed the presence of alkaloid, tannins, saponins and phenolic compounds and the absence of flavonoids and proteins. While, the aqueous extract showed the presence of alkaloids, flavonoids, saponins and phenolic compounds and the absence of tannin and proteins. In this evaluation, the highest zone of inhibition of the tested microorganism like P. aeruginosa, K. pneumonia, S. aureus, S. epididimis, C. krusei and C. tropicalis was observed in methanol extract (200µg/ml) of A. octandra leaves. The highest zone of inhibition was found against S. aureus and S. epididimis i.e., 25mm and 23 mm respectively at a concentration of 200µg/ml of methanol extract.
Conclusion: These outcomes showed the potential of the plant as a possible resource of bioactive constituents and present a scientific base for its ethnomedicinal uses for diseases. The results revealed that the methanol extract possesses better antimicrobial activity as compared to the aqueous extract and may be considered as an antimicrobial agent in the alternative treatment of multi-resistant pathogens.
Keywords: Phytochemicals, antimicrobial activity, Aporosa octandra, agar well diffusion, multi-drug resistance bacteria