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The drug evaluation and bioassay of traditional herbs of various herbal systems are now getting more momentum throughout the world. The present study provides information in respect of their identification, standardization of herbal drugs of the folk medicinal practice of present, and enrichment of Ayurvedic Pharmacopoeia. It is high time now to evaluate scientifically the information stored in different herbal medicine systems of the world in terms of their pharmacognostic and phytochemical characterization. So knowledge of the pharmacognosy of individual medicinal plants is a very important aspect for herbal-based drug discovery. Therefore in this study, an attempt has been made to evaluate such ethnomedicinally important plant, pharmacognostical and phytochemical analyses which are still unexplored. In this study, different pharmacognostical parameters of Ipomoea sagittifolia Burm.f. belonging to the family of Convolvulaceae, an ethnobotanically important medicinal plant, has been investigated. WHO recommended physico-chemical determinations and authentic phytochemical procedures. The physicochemical, morphological, and histological parameters presented in this paper may be proposed as parameters to establish the authenticity of the leaf of I. sagittifolia and may possibly help to differentiate the drug from its other species. In the leaf extract, the detected phytochemical groups are alkaloids, flavonoids, steroids, and triterpenoids, reducing sugars, tannins, gums, and saponins, etc. This study will be beneficial to herbalists and pharmacognostical and phytochemical for proper evaluation and validation of folk drugs. Herbal drugs used in various traditional medicine need detailed investigation with an ethnopharmacological approach.